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Time dependent changes of CD spectra of 4 μM kit2 induced by the addition of 150 mM KCl in 10 mM Tris pH 7.5, at (A) 10°C, (B) 25°C and (C) 37°C. Data were collected for 27 h. Arrows indicate the variation of the main peaks as a function of time, solid and dashed lines correspond to the oligonucleotide in the absence of metal ion and at the end of the process, respectively.
