Table 1. Distribution of the kit2 species in solution as derived by AUC analysis in 10 mM Tris pH 7.5 with/without 50 mM KCl and comparison of their experimentally determined sedimentation coefficients with those calculated by HydroPro software on solved structures.
Species in solution | Distribution in solution | Sedimentation coefficient (S) | |
---|---|---|---|
HydroPro | AUC | ||
Monomer | 60% | 1.50a | 1.52 |
Dimer | 40% | 2.53b | 2.65 |
Monomer (no KCl) | 100% | — | 1.47 |
aData calculated on PDB 2KYP.
bData calculated on PDB 2KYO.