Table 2. Kinetic constants derived by fitting the absorbance variation recorded at 295 nm after the addition of KCl using a ‘two kinetic processes’ model (Equation 1). Data were acquired in 10 mM Tris at 25°C.
[KCl] | [kit2] | k 1 (s-1) | k 2 (s-1) |
---|---|---|---|
10 mM | 6 μM | 6.00 ± 0.33 | 0.54 ± 0.04 |
50 mM | 6 μM | 6.96 ± 0.20 | 0.62 ± 0.03 |
50 mM | 4 μM | 8.13 ± 0.34 | 0.62 ± 0.04 |
50 mM | 2 μM | 8.12 ± 0.62 | 0.70± 0.09 |