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. 2017 Oct 23;45(22):13056–13067. doi: 10.1093/nar/gkx983

Table 2. Kinetic constants derived by fitting the absorbance variation recorded at 295 nm after the addition of KCl using a ‘two kinetic processes’ model (Equation 1). Data were acquired in 10 mM Tris at 25°C.

[KCl] [kit2] k 1 (s-1) k 2 (s-1)
10 mM 6 μM 6.00 ± 0.33 0.54 ± 0.04
50 mM 6 μM 6.96 ± 0.20 0.62 ± 0.03
50 mM 4 μM 8.13 ± 0.34 0.62 ± 0.04
50 mM 2 μM 8.12 ± 0.62 0.70± 0.09