Figure 7.

Stimulus-dependent RNAPII kinetics reflect the antagonistic and cooperative modules involved in the GC-to-GF crosstalk. (A) MCF10A cells were treated and ChIP-Seq of RNAPII was performed as in Figure 1. Shown is a bar graph presenting fold change of RNAPII binding of 45 module B genes following stimulation with EGF and DEX for 20 and 60 min, respectively, relative to starved cells. TSS and TES mark the TSS and end site, respectively. (B) Metagene graphs presenting fold change of RNAPII binding following stimulation with EGF, DEX and a combined stimulation (EGF + DEX) for 20 or 60 min. All module A (left side) and module B genes (right side) are presented.