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. 2017 Dec 13;5:17048. doi: 10.1038/boneres.2017.48

Figure 4.

Figure 4

Single vessel lengths, vessel density, sprout number, and connection number in ASC-paracrine angiogenesis are regulated by Wnt regulators in vitro. (a) 3D reconstruction showing the morphologies of vessel-like structures regulated by DKK1 and LiCl in ASC-EC co-cultured 3D collagen gels. The regulation of vessel-like structures by DKK1 and LiCl at 7 days (left) and 14 days (right) are shown to be representative of four different experiments (n=4). (bd) The parameter changes in ASC-paracrine angiogenesis by DKK1 and LiCl. The numbers shown in parentheses indicate images (vascular-like structure diameter (b) and single vessel length (c)) examined in each case, and similar results were observed for four independent experiments (n=4). The analysis of vessel density (d) was performed for least three independent experiments (n=3). *P<0.05; NS, no significant difference. (e) 3D reconstruction showing the sprouts and connections in vessel-like structures regulated by DKK1 and LiCl. The regulation of vessel-like structures by DKK1 and LiCl at 7 days (left) and 14 days (right) are shown to be representative of four different experiments (n=4). (f and g) Sprout number (e) and connection number (f) changes in ASC-paracrine angiogenesis by DKK1 and LiCl. The analyses of sprout number (f) and connection number (g) were performed for at least three independent experiments (n=3). *P<0.05; NS, no significant difference.