Table 3.
PCR primers.
| Gene | Locus | Description | PPrimer | Sequence (5′ → 3′) | Binding position | Product size (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|---|---|---|---|
| tpi | CD3172 | triosephosphate isomerase | tpi-Ftpi-R | GCAGGAAACTGGAAAATGCATAACAGATTGGCTCATATGCAACAAC | 18–505 | 488 | 55 | Lemèe et al.74 |
| groES | CD0193 | 10 kDa chaperone | groES-FgroES-R | TACCAGGAGCAGCTAAAGAGTATCTCCCACTGTCAATTCC | 80–187 | 108 | 55 | This study |
| groEL | CD0194 | 60 kDa chaperonin | groEL-FgroEL-R | ATACTGAATTAGATGCTGTTGAAGTCTGAAGTAGTTTGCTCTACTTG | 544–1069 | 526 | 53 | Lemèe et al.75 |
| fliC | CD0239 | flagellin subunit | fliC-FfliC-R | GGGGTTAGAATCAAGAGAGCGTTTTAGCTGCATCTGTTCC | 97–605 | 509 | 52 | This study |
| dnaJ | CD2460 | Chaperone protein | dnaJ-FdnaJ-R | TGGAAGAGCAAGAAGAAGAGTCAATCACTTCTCCAGTTCC | 339–623 | 285 | 54 | This study |
| dnaK | CD2461 | chaperone protein | dnaK-FdnaK-R | TGTTAGAAGGTGGAGAAGCAGTTGCTTGTCTTTGAGCATC | 53–389 | 337 | 56 | This study |
| grpE | CD2462 | heat shock protein | grpE-FgrpE-R | AAGCCGAATATGCAAACTACGATTTGCTTCAACTCCATCT | 242–541 | 300 | 54 | This study |
| hrcA | CD2463 | heat-inducible transcription repressor | hrcA-FhrcA-R | ATGAGTAAAAGCGAATTGGATTGTCATTCATAGCAACCAA | 238–440 | 202 | 55 | This study |