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. 2017 Dec 13;7:17511. doi: 10.1038/s41598-017-17787-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spreading behaviour and viability of iDCs are not affected by substrate stiffness. (a) Representative brightfield images taken with a 40x objective of iDCs in culture on PAA substrates of 2, 12 or 50 kPa after 3 days of differentiation. (b) Viability of iDCs after 6 days of differentiation on PAA substrates of 2, 12, or 50 kPa (n = 6 donors), represented as the eFluor780-negative fraction of cell population measured by flow cytometry. Bars represent mean with SEM.