Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 13;7:17511. doi: 10.1038/s41598-017-17787-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Substrate stiffness regulates C-type lectin expression on iDCs. (a,b) Cell surface expression of C-type lectins MMR (a, n = 7 donors) and DC-SIGN (b, n = 7 donors) on iDCs conditioned for 6 days on PAA substrates of 2, 12 and 50 kPa, represented as geometric mean fluorescence intensity measured with flow cytometry. Bars represent mean with SEM. Statistical significance was tested with repeated measures ANOVA. (c,d) Representative images of western blot analysis of total MMR protein expression (c) and total DC-SIGN protein expression (d) in iDCs conditioned for 6 days on PAA substrates of 2, 12 or 50 kPa. Unprocessed original scans of blots are shown in Supplementary Fig. S9. (e,f) Quantification of integrated intensity of total MMR protein expression (e, n = 3 donors) and total DC-SIGN protein expression (f, n = 3 donors) measured by Western Blot analysis in iDCs conditioned for 6 days on PAA substrates of 2, 12 or 50 kPa. Per donor, integrated intensities are normalized to 2 kPa results. Bars represent mean with SEM. Statistical significance was tested with repeated measures ANOVA. *p < 0.05; ***p < 0.001.