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. 2017 Dec 13;7:17498. doi: 10.1038/s41598-017-17888-9

Figure 1.

Figure 1

Down-stream signalling of the unfolded protein response (UPR) is enhanced in metastatic melanoma cell lines. (a) Illustration of the UPR and selected down-stream targets of the ATF6, PERK and IRE1 pathway. (b) Isogenic non-metastatic and metastatic melanoma cells and primary melanocytes were cultivated in MIM and protein expression was analyzed by immunoblotting. One representative blot out of three independent experiments is shown. Uncropped immunoblot scans are shown in supplementary Fig. S7. (c–e) mRNA expression pattern of down-stream targets of the UPR in isogenic MCM1G and MCM1DLN and (f–h) isogenic WM793B and 1205Lu cells. Cells were cultivated in MIM, expression was determined by RT-qPCR and results were normalized to β-actin. Pooled data of at least three independent experiments are shown.