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. 2017 Dec 13;7:17487. doi: 10.1038/s41598-017-17832-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hsp90 inhibition decreases oxidative and glycolytic metabolism in Her2-overexpressing and triple negative breast cancer. A seahorse extracellular flux analyzer was used to determine the baseline and post-treatment metabolic properties of BT-474, MCF-7, and MDA-MB-231 cells. The endpoints monitored were oxygen consumption rate (OCR, left column) and extracellular acidification rate (ECAR, right column). (a) Baseline OCR for each cell line. (b) Baseline ECAR for each cell line. (c) OCR or (d) ECAR for each cell line after 12, 24, or 48-hour treatment with 100 µM HS-27 or DMSO (vehicle). (e) MDA-MB-231, MCF-7, and BT-474 cells were plated at uniform density and treated with either 100 µM HS-27 or DMSO control for 48 hours. ANOVA with Tukey-Kramer post hoc testing (n = 6) was used for (a) and (b), while two-sided t-tests (n = 6) were used for (c) and (d) (n = 3) for (e).