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. 2017 Dec 13;7:17496. doi: 10.1038/s41598-017-17406-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kv3.1 mRNA is expressed in the inferior colliculus and auditory cortex, even after profound contralateral auditory nerve damage. Top row, inferior colliculus. Bottom row, auditory cortex. (a) Individual KCNC1 mRNA transcripts that encode the Kv3.1 protein identified near DAPI-labeled nuclei of individual cells. Fluorescently labeled individual mRNA transcripts are identified within a fixed radius of each nucleus, counted, and then assigned to a given cell with automated software. Scale bars = 3 μm. (b) The percentage of DAPI + nuclei within a 185 μm² region of interest that contain at least 10 mRNA transcripts are quantified in the ICc (top) or ACtx (bottom) 30 days after control or ouabain treatment (n = 36 imaging regions from 4 ouabain-treated mice and 27 imaging regions from 3 sham-treated mice). (c) Same as B, except the number of identified transcripts are quantified only in the Kv3.1+ cells (n = 2,420/2,814 in the ICc and 941/1,399 cells in ACtx for ctrl/ouabain, respectively). Plots in 5B and 5 C depict the median, interquartile range and 95% confidence intervals. Asterisk indicates p < 0.05 with a rank sum test; otherwise, pairwise differences between control (Ctrl) and ouabain are not significant.