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. 2017 Dec 13;7:17484. doi: 10.1038/s41598-017-17459-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-lapse imaging analysis of the cell surface pH using FITC-PEG-lipid. KATOIII cells labeled with FITC-PEG-lipids were subjected to a time-lapse imaging analysis. Images were acquired at the excitation wavelengths of 458 nm and 488 nm, with the fluorescence detection range of 500–550 nm. The external solutions were seamlessly switched by using a stage-top perfusion system in the following order: pH 6.5, 6.0, 5.5 and recovery to pH 6.5. The pH values of the perfused external solutions are shown above the psudocolored fluorescence ratio images. Interval: 10 sec. The total duration: 6 min 20 sec (39 frames). Scale bar: 10 μm.