Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 13;7:17525. doi: 10.1038/s41598-017-17758-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Immunofluorescence analyses following TBI and PLX5622 treatment. (A) Representative immunofluorescence images taken at 7 d post-injury showing a strong reduction of microglia/macrophages (Iba-1, red; CD11b, green), and CD68 expressing microglia/macrophages (yellow) in the group that received the PLX5622 compared to control diet. Quantitative analyses in the injured cortex confirmed decrease of (B) Iba-1 expressing microglia/macrophage population (92 ± 7%, p < 0.0001), (C) CD11b expressing microglia/macrophages (83 ± 5%, p = 0.0001), (D) the lysosomal marker CD68 (76 ± 4%, p = 0.0014), in the group that received the PLX5622 diet. Similarly, quantitative analyses in the contralateral cortex showed decrease of (E) Iba-1 and (F) CD11b expressing microglia/macrophage population (84 ± 7%, p < 0.0082 and 87 ± 6%, p < 0.0019, respectively) for mice that received PLX5622 compared to control diet, while the (G) lysosomal marker CD68 levels were below detection. All values are reported as mean ± sem (n = 3–5 mice per group).