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. 2017 Dec 13;7:17483. doi: 10.1038/s41598-017-16997-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Double-reference SPR sensorgrams obtained by flowing solutions of helional at 2 µM (data in violet), 3 µM (data in green) and 5 µM (data in orange) onto a L1 chip with OR17-40-NV (flow rate: 60 µL/min). The association phase was followed for 180 s and the dissociation phase was followed for 620 s. Inset: Corresponding sensorgrams obtained by flowing through the same chip heptanal solutions at the same concentrations. (B) Double-subtracted SPR sensorgrams obtained by flowing solutions of androstenone at 1 µM (data in violet), 5 µM (data in green) and 10 µM (data in orange) onto a L1 chip with OR7D4-NV (flow rate: 60 µL/min).