Table 1.
Primary and secondary antibodies used in the current experiments.
| Primary antibody | Application | Source/Country |
|---|---|---|
| anti-LC3B (rabbit polyclonal) | immunoblot | ab48394, Abcam/Japan |
| immunohistochemistry | ||
| anti-cathepsin D (rabbit polyclonal) | immunoblot | ab826, Abcam/Japan |
| immunohistochemistry | ||
| anti-p62 (SQSTM1) (rabbit polyclonal) | immunoblot | PM045, MBL/Japan |
| anti-SIRT1 (H-300) (rabbit polyclonal) | immunoblot | sc-15404, SantaCruz /USA |
| anti-SIRT3 (rabbit polyclonal) | immunoblot | ab75434, Abcam/Japan |
| anti-ATPB antibody (3D5) (mouse monoclonal) (mitochondrial ATP synthase β subunit protein) |
immunoblot | ab14730, Abcam/Japan |
| immunohistochemistry | ||
| anti-SOD2/MnSOD (rabbit polyclonal) | immunoblot | ab13534, Abcam/Japan |
| immunohistochemistry | ||
| anti-Akt(pan) (C67E7) (rabbit polyclonal) | Immunoblot | 4691, Cell Signaling Technology/Japan |
| anti-phospho-Akt (Ser473) (D9E) (rabbit polyclonal) | immunoblot | 4060, Cell Signaling Technology/Japan |
| anti-AMPKα1 + AMPKα2 (rabbit polyclonal) | immunoblot | ab39644, Abcam/Japan |
| anti-phospho-AMPKα (Thr172) (40H9) (rabbit monoclonal) | immunoblot | 2535, Cell Signaling Technology/Japan |
| anti-mTOR (rabbit polyclonal) | immunoblot | 2972, Cell Signaling Technology/Japan |
| anti-phospho-mTOR (Ser2448) (D9C2) (rabbit monoclonal) | immunoblot | 5536, Cell Signaling Technology/Japan |
| anti-synaptophysin1 (rabbit polyclonal) | Immunoblot Immunohistochemistry |
101002, Synaptic Systems/Germany |
| anti-neurofilament H (NF-H), Nonphosphorylated (SMI32) (mouse monoclonal) | immunoblot | 801701, BioLegend/USA |
| immunohistochemistry | ||
| anti-MAP2A, 2B, 2C (mouse monoclonal) | immunoblot | MAB364, Chemicon/USA |
| immunohistochemistry | ||
| anti-desmin (mouse monoclonal) | immunoblot immunohistochemistry |
M0760, Dako/Japan |
| anti-GAPDH (rabbit polyclonal) | immunoblot | NB100-56875, Novus Biologicals/USA |
| Secondary antibody | ||
| anti-mouse immunoglobulins (goat polyclonal) | immunoblot | P0477, Dako/Japan |
| anti-rabbit immunoglobulins (swine polyclonal) | immunoblot | P0399, Dako/Japan |
| anti-mouse IgG(H + L) AlexaFluor 488 (goat polyclonal) | immunohistochemistry | A-11001, ThermoFisher/Japan |
| anti-rabbit IgG(H + L) AlexaFluor 488 (goa polyclonal) | immunohistochemistry | A-11008, ThermoFisher/Japan |
| anti-mouse IgG(H + L) AlexaFluor 555 (goat polyclonal) | immunohistochemistry | A-21422, ThermoFisher/Japan |
| anti-rabbit IgG(H + L) AlexaFluor 555 (goat polyclonal) | immunohistochemistry | A-21428, ThermoFisher/Japan |