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. 2017 Oct 23;14(6):8114–8121. doi: 10.3892/ol.2017.7250

Figure 3.

Figure 3.

Inhibition of DDR2 reverses TGF-β- and collagen I-induced EMT in TPC-1 cells. (A) TPC-1 cells were transfected with DDR2 siRNA. The knockdown efficiency was detected by RT-qPCR. (B) The interfering efficiency of DDR2 siRNA was verified by western blotting. (C) Western blotting results of E-cadherin and Vimentin in TPC-1 cells transfected with DDR2 siRNA or control siRNA. (D) TPC-1 cells transfected with DDR2 siRNA or control siRNA were incubated with 5 ng/ml of TGF-β for 48 h. The E-cadherin and Vimentin protein level was detected by western blotting. (E) TPC-1 cells transfected with DDR2 siRNA or control siRNA were incubated with 2 µg/ml collagen I for 48 h. The E-cadherin and Vimentin protein level was detected by western blotting. **P<0.01. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; DDR2, discoidin domain receptor tyrosine kinase 2; si, small interfering; TGF, transforming growth factor.