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Primer sequence of RT-PCR
| Primer name | Base sequence 5-3 | Gene order |
|---|---|---|
| Collagen II | F:GAGGGCAACAGCAGGTTCAC | NM_012929.1 |
| R:GCCCTATGTCCACACCAAATTC | ||
| Collagen II | F:CATGCCGTGACCTCAAGATG | NM_053304.1 |
| R:TCCATCGGTCATGCTCTCTC | ||
| Aggrecan | F:TGGCATTGAGGACAGCGAAG | NM_022190.1 |
| R:TCCAGTGTGTAGCGTGTGGAAATAG | ||
| β-actin | F:TTCAACACCCCAGCCATGTA | NM_031144.3 |
| R:CAGGAAGGAAGGCTGGAAGA | ||
| SOX-9 | F:GAAAGACCACCCCGATTACAAG | XM_003750950.3 |
