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Foam nests of the túngara frog (E. pustulosus). (A) Nesting pair in amplexus. (B) Colony nests in a temporary pool (Lopinot valley, Trinidad). (C) Single nest, ca. 10 cm diameter, sliced through (D) showing the distribution of eggs (ca. 400) within the foam. (E) SDS gel electrophoresis analysis of foam fluid showing six major protein components (ranaspumins), standard molecular weight (kDa) markers to the left. (Picture credits: Alan Cooper, Rachel Fleming).
