Table 2.
Significant differences in gene expression between the biopaper and PET coculture systems at each time point. Genes with significantly higher expression on the biopaper and PET are shown in italic and bold formats, respectively. Genes with no expression on the biopaper or PET at any time are presented with dashes.
| Day 3 | Day 7 | Day 14 | Day 21 | Day 28 | ||
|---|---|---|---|---|---|---|
| Extracellular matrix-associated proteins | SPARC | p = 0.003 | p = 0.022 | |||
| COL4A1 | p = 0.003 | |||||
| LAMA1 | p = 0.02 | |||||
| LAMA2 | p = 0.006 | |||||
| LAMA4 | p = 0.018 | p = 0.018 | ||||
| AGRN | ||||||
|
| ||||||
| Functional astrocyte proteins | GFAP | |||||
| AQP4 | ||||||
|
| ||||||
| Membrane transporters and soluble factors | PDGFB | p < 0.001 | p = 0.004 | p = 0.007 | p < 0.001 | |
| PDGFRB | p < 0.001 | p = 0.004 | p = 0.01 | |||
| GGT5 | p = 0.002 | p = 0.004 | ||||
| LIF | p = 0.018 | p = 0.045 | ||||
| FGF2 | p = 0.029 | |||||
|
| ||||||
| Endothelial tight junction transmembrane proteins | OCLN | |||||
| CLDN1 | p = 0.047 | |||||
| CLDN3 | — | — | — | — | — | |
| CLDN5 | ||||||
| CLDN12 | p = 0.003 | |||||
|
| ||||||
| Endothelial adherens junction transmembrane proteins | CDH5 | p = 0.02 | p < 0.001 | p = 0.001 | p < 0.001 | |
|
| ||||||
| Endothelial junction accessory proteins | TJP1 | p = 0.008 | ||||
| TJP2 | p < 0.001 | p = 0.001 | p = 0.03 | p < 0.001 | ||
| TJP3 | — | — | — | — | — | |
|
| ||||||
| Gap junction proteins | GJA1 | p = 0.049 | ||||
| GJB4 | — | — | — | — | — | |
| GJB6 | — | — | — | — | — | |
|
| ||||||
| Cell adhesion proteins | VCAM1 | p < 0.001 | p = 0.02 | |||
| PECAM1 | p < 0.001 | p = 0.048 | ||||