Figure 1.

Generation of SNAP-25 forebrain-specific KO mice. (a) Targeting strategy used for the deletion of the SNAP-25 exon 4. P1-2 referred to PCR primers for genotyping, which is located on intron flank exon 4 separately. (b) PCR genotyping of recombinant SNAP-25 locus. (c) Western blot analysis of brain extracts of Ctrl and mutant mice. Right panel: quantitative analysis of western blot images. n = 3 per group. ^∗∗∗P < 0.001 compared with control littermates. (d–k) Immunostaining with anti-SNAP-25 (red) and anti-VGLUT1 (green) of cortex of sagittal sections from adult mice brains. Scale bars are 20 μm in (d) and 200 μm in (e).