Skip to main content
. 2017 Nov 26;2017:3619879. doi: 10.1155/2017/3619879

Figure 5.

Figure 5

Ta-EE exerted a chondroprotective effect by downregulating MMP gene expression rather than upregulating the gene expressions of COL2A1 and CHSY1 in SW1353 cells. (a) SW1353 cells were treated with Ta-EE (0–300 μg/ml) for 24 h, and cell viability was measured by MTT assay. (b) SW1353 cells pretreated with Ta-EE (0–300 μg/ml) for 30 min were treated with PMA (100 nM) for 6 h, and the mRNA expression levels of MMP1, MMP2, MMP9, and MMP13 in the cells were measured by semiquantitative PCR. SW1353 cells pretreated with Ta-EE (300 μg/ml) for 30 min were treated with PMA (100 nM) for the indicated time, and the total and phosphorylated protein levels of (c) IKKα/β and IκBα and (d) ERK, JNK, and p38 in the total cell lysates were determined by Western blotting. β-Actin was used as an internal control. (e) SW1353 cells were treated with Ta-EE (0–300 μg/ml) for 6 h, and the mRNA expression levels of COL2A1 and CHSY1 in the cells were measured by quantitative real-time PCR.