Table 1.
Target | Sequence (5′ to 3′) | |
---|---|---|
Semiquantitative real-time PCR | ||
MMP1 | Forward | CCCAGCGACTCTAGAAACACA |
Reverse | CTGCTTGACCCTCAGAGACC | |
MMP2 | Forward | ACGACCGCGACAAGAACTAT |
Reverse | CTGCAAAGAACACAGCCTTCTC | |
MMP9 | Forward | CAGTACCGAGAGAAAGCCTA |
Reverse | ACTGCAGGATGTCATAGGTC | |
MMP13 | Forward | GAAATGCAGTCTTTCTTCGG |
Reverse | GCCTTTTCGACTTCAGAATG | |
GAPDH | Forward | CACTCACGGCAAATTCAACGGCAC |
Reverse | GACTCCACGACATACTCAGCAC | |
| ||
Quantitative real-time PCR | ||
IL-1β | Forward | TAGAGCTGCTGGCCTTGTTA |
Reverse | ACCTGTAAAGGCTTCTCGGA | |
iNOS | Forward | GGAGCCTTTAGACCTCAACAGA |
Reverse | TGAACGAGGAGGGTGGTG | |
COX-2 | Forward | CACTACATCCTGACCCACTT |
Reverse | ATGCTCCTGCTTGAGTATGT | |
COL2A1 | Forward | GCAACGTGGTGAGAGAGGAT |
Reverse | CCTGTCGTCCGGGTTCAC | |
CHSY1 | Forward | ATTGTCATGCAGGTCATGGA |
Reverse | CTCACAGGGACCGTCATTTT | |
GAPDH | Forward | CAATGAATACGGCTACAGCAAC |
Reverse | AGGGAGATGCTCAGTGTTGG |