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. 2017 Aug 8;1(18):1440–1451. doi: 10.1182/bloodadvances.2017007591

Figure 6.

Figure 6.

RUNX family members transactivate GSTA2 expression. (A) Expressions of RUNX1, RUNX2, RUNX3, and total RUNX (Pan_RUNX) were determined in MV4-11 cells transduced with control or with expressing vectors of RUNX1, RUNX2, and RUNX3 in the presence of 3 μM of doxycycline for 24 h. Values are normalized to those of control vector-transduced cells (n = 3). (B) Expressions of GSTA2 were determined in MV4-11 cells as in panel A. Values are normalized to those of control vector-transduced cells (n = 3). (C) Expressions of RUNX1, RUNX2, RUNX3, GSTA2, and GAPDH were determined in MV4-11 cells transduced with control or with expressing vectors of RUNX1, RUNX2, and RUNX3 in the presence of 3 μM of doxycycline for 48 h (n = 3). (D) Luciferase reporter assay of GSTA2 promoter. HEK293T cells were transduced with the indicated lentivirus vectors as well as with luciferase reporter plasmids and then incubated with 3 μM of doxycycline. Forty-eight hours after treatment, relative luciferase activity was determined (n = 3). (E) Growth curves of MV4-11 cells as in panel A. Cells were cultured in the presence of 3 μM of doxycycline (n = 3). Data are mean ± SEM values. *P < .05, by 2-tailed Student t test; **P < .01.