Skip to main content
. Author manuscript; available in PMC: 2018 May 13.
Published in final edited form as: Nat Med. 2017 Nov 13;23(12):1454–1465. doi: 10.1038/nm.4429

Figure 4.

Figure 4

UCP1-independent roles of beige fat in the regulation of body-weight and glucose metabolism in vivo. (a) Body-weight of Prdm16 Tg mice and the littermate controls under HFD at 22°C. n = 7 for both groups. *P < 0.05. (b) Body-weight of Prdm16 Tg x Ucp1−/− and the littermate Ucp1−/− mice under HFD at 22°C. Ucp1−/−, n = 8; Prdm16 Tg x Ucp1−/−, n = 12. *P < 0.05. (c) Adiposity (fat mass per body-weight) of mice at 18 weeks of HFD. Control, n = 7; Prdm16 Tg, n = 7; Ucp1−/−, n = 8; Prdm16 Tg x Ucp1−/−, n = 12. ***P < 0.001. n.s., not significant. (d) GTT in Prdm16 Tg and the littermate controls (left) and Prdm16 Tg x Ucp1−/− and the littermate Ucp1−/− mice (right) at 10 weeks of HFD at 22°C. Control, n = 9; Prdm16 Tg, n = 8; Ucp1−/−, n = 10; Prdm16 Tg x Ucp1−/−, n = 7. *P < 0.05, **P < 0.01, ***P < 0.001. (e) ITT in mice in (d) at 11 weeks of HFD. *P < 0.05. (f) Body-weight of Prdm16 Tg and the littermate controls (left) and Prdm16 Tg x Ucp1−/− and the littermate Ucp1−/− mice (right) under HFD at 30°C. Control, n = 9; Prdm16 Tg, n = 7; Ucp1−/−, n = 7; Prdm16 Tg x Ucp1−/−, n = 8. *P < 0.05. (g) GTT in Prdm16 Tg and the littermate controls (left) and Prdm16 Tg x Ucp1 −/− and the littermate Ucp1−/− mice (right) at 10 weeks of HFD under 30°C. Control, n = 9; Prdm16 Tg, n = 7; Ucp1−/−, n = 7; Prdm16 Tg x Ucp1−/−, n = 5. *P < 0.05, ***P < 0.001. Data are expressed as means ± s.e.m. Data analyzed by Student’s t-test (c) and ANOVA followed by Fisher’s LSD test (a,b,d–g).