Skip to main content
. 2017 Jul 25;1(17):1335–1346. doi: 10.1182/bloodadvances.2017008359

Figure 4.

Figure 4.

Intestinal Heph is not essential for the enhancement of iron absorption after induction of acute hemolysis. Adult mice were injected with the hemolytic agent (PHZ) to stimulate erythropoiesis. Five days later, iron absorption and utilization were determined. Hb levels were measured from blood samples taken at euthanization (A). Iron (59Fe) absorption (B) and tissue distribution (C-F) were quantified by well-established methods. Data are shown as box-and-whisker plots and were analyzed by 3-way ANOVA. No significant 3-way interactions were noted. A PHZ main effect was noted for all measured parameters (P < .0001 for all). A sex main effect was noted for 59Fe absorption (P = .0068) and for iron accumulation in blood, liver, and bone (P < .011 for all), but no genotype main effects were observed for these parameters. PHZ × sex interactions were noted for blood (P = .0016) and spleen (P = .0107) 59Fe accumulation, and a genotype × sex interaction was observed for 59Fe absorption (P = .0419). The number of mice in each group is indicated in parentheses beneath panel A and is the same for the data shown in all panels, unless otherwise indicated.