Figure 6.

Flagellin-induced MPP3 cells are myeloid biased and predominantly differentiate into neutrophils. Two thousand MPP3 cells were FACS-sorted from whole bone marrow cells of flagellin-treated CD45.2⁺ mice and transferred to irradiated CD45.1⁺ WT B6 mice along with 4 × 10⁵ whole bone marrow cells from CD45.1⁺ mice. Whole blood cells of recipient mice were analyzed at indicated time points by flow cytometry using antibody to CD45.1, CD11b, and Ly6G to show peripheral blood cells differentiated from MPP3. Representative flow cytometry plots of MPP3-originated myeloid cells (CD45.1⁻CD11b⁺) and neutrophils (CD45.1⁻CD11b⁺Ly6G⁺) are shown.