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. 2017 Oct 25;29(11):2817–2830. doi: 10.1105/tpc.17.00371

Figure 1.

Figure 1.

Light Induces ICE Accumulation.

(A) Light-induced accumulation of ICE proteins. Four-day-old ProICE1:MYC-ICE1 and ProSCRM2:MYC-SCRM2 transgenic seedlings grown on MS-agar plates in darkness were exposed to different light wavelengths for 12 h before extracting total proteins from whole seedlings. ICE proteins were detected immunologically using an anti-MYC antibody. Tubulin (TUB) was detected similarly as the loading control. WL, white light; D, darkness; B, blue light; R, red light; FR, far-red light. Blots on the membranes were quantitated using ImageJ software.

(B) Kinetics of ICE1 accumulation. Four-day-old 35S:MYC-ICE1 transgenic seedlings grown on MS-agar plates in darkness were exposed to different light wavelengths for up to 12 h before extracting total proteins from whole seedlings. Immunological detection of ICE1 was performed as described above. Blots on the membranes were quantitated as described above. Quantitations of three blots from independent biological samples were averaged. Bars indicate se of the mean.