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. 2017 Oct 23;29(11):2854–2870. doi: 10.1105/tpc.17.00438

Figure 2.

Figure 2.

WRKY75 Positively Regulates Leaf Senescence in an Age-Dependent Manner.

(A) The senescence phenotype of 40-d-old Col-0, WRKY75-knockdown plants obtained via RNAi (WRKY75RNAi), and WRKY75 overexpressing plants (WRKY75ox). Two independent lines of each transgenic plant were examined.

(B) The fourth rosette leaves detached from whole plants at the indicated ages.

(C) qRT-PCR analysis of WRKY75 expression in 7-d-old Col-0, WRKY75RNAi, and WRKY75ox plants. Student’s t test, **P < 0.01 and ***P < 0.001. Data are represented as means ± sd, n = 3. The experiment was performed three times with similar results.

(D) and (E) Chlorophyll contents (D) and SAG12 expression (E) in the leaves shown in (B). WRKY75RNAi #1 and WRKY75ox #1 were used for all experiments involving the two types of transgenic plants in subsequent analyses. Data are represented as means ± sd, n = 3. The experiments were performed three times with similar results.