WRKY75 Is Involved in SA Biosynthesis and Plant Defense Responses.
(A) Measurement of free SA levels in the third and fourth rosette leaves from 32-d-old plants. Student’s t test, *P < 0.05. Data are represented as means ± sd, n = 3.
(B) qRT-PCR analysis of transcript levels of PR1 and PR5 in leaves (A). Student’s t test, *P < 0.05 and **P < 0.01. Data are represented as means ± sd, n = 3. The experiment was performed three times with similar results.
(C) Phenotypes of 3-week-old Col-0, wrky75-c2, WRKY75RNAi, and WRKY75ox leaves inoculated with DC3000 bacteria for 3 d. The experiment was performed twice, with similar results.
(D) The bacterial populations in inoculated leaves shown in (C). Student’s t test, *P < 0.05 and **P < 0.01. Data are represented as means ± sd, n = 8.