Mutation of SID2 Suppresses the Early Leaf Senescence Phenotype of WRKY75ox Plants.
(A) qRT-PCR analysis of WRKY75 and SID2 expression in the third and fourth rosette leaves from 28-d-old plants. Student’s t test, **P < 0.01 and ***P < 0.001. Data are represented as means ± sd, n = 3. The experiment was performed three times with similar results.
(B) Measurement of free SA levels in the leaves shown in (A). Student’s t test, **P < 0.01 and ***P < 0.001. Data are represented as means ± sd, n = 3.
(C) The senescence phenotypes of 28-d-old plants with the indicated genotypes.
(D) Chlorophyll contents in Col-0, WRKY75ox, WRKY75ox/sid2-2, and sid2-2 leaves. The third and fourth rosette leaves were used. Data are represented as means ± sd, n = 3. The experiment was performed three times with similar results.