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. 2017 Oct 17;29(11):2801–2816. doi: 10.1105/tpc.17.00645

Figure 2.

Figure 2.

Expression of OsMADS14 and OsMADS15 in Leaves Is Dependent on Expression of Hd3a and RFT1.

(A) Schematics of the inducible system used in this study. The GVG chimeric protein is expressed under the GOS2 promoter to produce the inducible part of the vector. The Hd3a or RFT1 coding sequences are cloned under the control of the 4x UPSTREAM ACTIVATION SEQUENCE (UAS) to produce the effector component of the vector. T indicates the terminator.

(B) to (E) Expression of Hd3a (B), RFT1 (C), OsMADS14 (D), and OsMADS15 (E) in leaves of DEX-inducible transgenic plants grown under LD. Leaves were harvested at ZT0. GVG:Hd3a and GVG:RFT1 indicate DEX-inducible Hd3a- and RFT1-overexpressing lines, respectively. Two independent transgenic lines are shown for each construct. Plants were either DEX- or mock-treated, and transcripts were quantified using primers designed on the coding sequences. UBQ was used as standard for quantification of gene expression. Data are represented as mean ± sd. xE-n = × 10−n. ANOVA tests for graphs in (B) to (E) are shown in Supplemental File 1.