Table 2.
Primers and probes
| Primers and probes studied | Primer and probe sequences |
|---|---|
| TaqMRPS19F | TGGTGGAAAAGGACCAAGATG |
| TaqMRPS19R | CCGGCGATTCTGTCCAGAT |
| ProbeRPS19 | CCGCAAACTGACACCTCAGGGACA |
| TaqMRPL5F | CTGTGGATGGAGGCTTGTC |
| TaqMRPL5R | CATTAAATTCCTTGCTTTCAGAATCA |
| Probe RPL5 | ATCCCTCACAGTACCAAACGATTCCCTGG |
| TaqMRPL11F | TTGGTATCTACGGCCTGGACTT |
| TaqMRPL11R | GATTCTGTGTTTGGCCCCAAT |
| ProbeRPL11 | TTTCAGCATCGCAGACAAGAAGCGC |
| TaqMhup53F | TTTGCGTGTGGAGTATTTGGAT |
| TaqMhup53R | TGTAGTGGATGGTGGTACAGTCAGA |
| ProbeHup53 | CACTTTTCGACATAGTGTGGTGGTGCCCTA |
| TaqMHSP70huF | ACATGAAGCACTGGCCTTTC |
| TaqMHSP70huR | CTCGGCGATCTCCTTCATCT |
| ProbehuHSP70 | AGCTCACCTGCACCTTGGGCT |
| TaqMhuGATA1F | CCTCATCCGGCCCAAGA |
| TaqMhuGATA1R | TGGTCGTCTGGCAGTTGGT |
| ProbeGATA1hu | TGATTGTCAGTAAACGGGCAGGTAC |
| TaqMhuGAPDHF | CCTGTTCGACAGTCAGCCG |
| TaqMhuGAPDHR | CGACCAAATCCGTTGACTCC |
| ProbeGAPDHhu | AGCCACATCGCTCAGACACCATGG |
| TaqMHPRThuF | GGCAGTATAATCCAAAGATGGTCAA |
| TaqMHPRThuR | TCAAATCCAACAAAGTCTGGCTTATAT |
| ProbeHPRThu | CTTGCTGGTGAAAAGGACCCCACGA |
| TaqMB2MhF | GCGGCATCTTCAAACCTCC |
| TaqMB2MhR | TGACTTTGTCACAGCCCAAGATA |
| ProbeB2Mh | TGATGCTGCTTACATGTCTCGATCCCACTT |
Total RNA was extracted using TRIzol Reagent and treated with RNase-Free DNase. Reverse transcription was carried out with 500 ng total RNA. Real-time PCR was performed using the ABI 7900 Real-time PCR system and TaqMan PCR mastermix (Life Technologies). Quantification of gene amplification was performed in duplicate using the ΔΔCt method. The expression level of each gene was normalized using 3 housekeeping genes: hypoxantine-guanine-phosphoribosyl-transferase (HPRT) gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, and β2 microglobulin gene. Primers and probes are listed.