Figure 1.

PF-06747143 blocks CXCL12-induced pathways. (A) cAMP assay was performed in CHO-K1 cells transfected with hCXCR4 (CHO-K1-CXCR4), incubated with CXCL12 at its EC₈₀ (1.25 nM) and PF-06747143 or IgG1 control Ab. Experiments were performed in triplicates. Bars represent standard error of the mean (SEM). (B) Migration assay was performed in Ramos human NHL cells incubated in transwell chambers for 24 hours with PF-06747143 or IgG1 control Ab. CXCL12 (100 ng/mL) was used a chemoattractant in the bottom chamber. Data are shown as mean % migration inhibition. Experiment was performed in quadruplicates. Bars represent SEM.