Figure 4.

Elevated embedded rGMPs in mtDNA of brain and heart from Mpv17 null mice, without altered dNTP pools or mtDNA copy number. (A) Mitochondrial (mt) DNA copy number in brain and heart of Mpv17^−/− mice (gray) relative to controls (WT), n = 3 independent experiments, t-test; and (B) Brain mitochondrial dNTP pools in control (black) and Mpv17^−/− (gray) animals of 8–10 weeks of age. P values were obtained using Mann-Whitney test (n = 4). Not significant (ns) P > 0.05. Data are expressed as mean ± SEM. (C) Relative frequency of incorporated ribonucleotides of heart and brain mtDNA of Mpv17 KO mice of 2–3 months of age determined by emRiboSeq represented as pie charts; rA – rAMP, rC – rCMP, rG – rGMP, rU – rUMP/rTMP.