Figure 5.

Multiple deletions that are synonymous with DNA replication stalling are detected in the brain of Mpv17 null mice of 12 months of age. (A) Southern blot of brain DNA of mice aged 1 year hybridized with a probe to np 14881–16299 of the murine mitochondrial genome. Wild-type mice (WT) and Mpv17 knockout animals (KO). Note the more prominent smear of linear deleted molecules below the full length mtDNAs in the KO samples. (B) dNTP levels in brain mitochondria of Mpv17 KO mice aged 2–3 months or 10–12 months, expressed relative to age matched controls, n = 4. P values were obtained using Mann-Whitney test (NS, not significant P>0.05).