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. 2017 Nov 7;45(22):12848–12861. doi: 10.1093/nar/gkx1056

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Changes in I-SceI flanking sequence alter the deletion boundaries. Deletion boundaries for inaccurate repair events for (A) Iw7, (B) M1, (C) M2 and (D) M3. Deletion boundaries are defined as the first non-ambiguous base accurately aligned to the left and right of the I-SceI break. SD-MMEJ consistent junctions are represented by darker colors. + indicates all bases to the right or left of the indicated sequence. The arrow in (B) shows the effect of the C→A mutation on the deletion boundary at a prominently used primer repeat.