Figure 7.

The prevalence of repeat motifs utilized in SD-MMEJ consistent single-step insertions depends on the I-SceI flanking sequence. Repeat motifs contain the P1 primer repeat, the adjacent MH1 microhomology repeat, and any DNA between these repeats (the insertion). Colors correspond to the frequency that each base in found in a repeat motif, with warmer colors indicating greater frequencies. Red vertical lines indicate the TTAT/AATA overhangs produced by I-SceI cutting. Trans SD-MMEJ occurs with microhomology annealing across the break site, followed by synthesis, dissociation of the nascent DNA, and reannealing at new microhomologies prior to completion of repair.