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. 2017 Nov 7;45(22):12834–12847. doi: 10.1093/nar/gkx1047

Figure 3.

Figure 3.

Dynamic behavior of PARP1 on DNA containing abasic sites. (A) Bar graph showing PARP1 behavior on DNA containing AP sites (N = 129, M: Motile, D: Dissociated, M & D: Motile and Dissociated). Data from three independent experiments are shown as weighted mean ± SEM. Motile population (M) includes protein particles of all three types of movement on DNA (random, constrained, and paused). (B) Kymographs showing four different types of motion of PARP1: (I) random, (II) constrained, (III) paused, and (IV) non-motile. Horizontal and vertical scale bars represent 5 seconds and 2 kbp, respectively. (C) Bar graph showing types of motion of PARP1 on AP DNA. Data from three independent experiments are shown as weighted mean ± SEM. Note that the y-axis breaks between 10% and 80%. (D) Plot of PARP1 anomalous diffusion exponent (α factor) versus diffusion coefficient (log10D).