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. 2017 Nov 16;45(22):12601–12610. doi: 10.1093/nar/gkx1129

Figure 4.

Figure 4.

Effect of tRNA structure on the EF-P-dependent acceleration of d-amino acid incorporation. (A) Structure of tRNAs used for d-amino acid incorporation. Red and blue letters in tRNAPro1E1CGG and tRNAPro1E2CGG indicate alteration from the wild-type tRNAPro1CGG sequence. (B) Expression level of peptide rP1 containing two consecutive d-Ala at CCG codons. tRNA used for d-Ala incorporation were indicated. Numbers above the bars indicate the ratio of EF-P(+) to EF-P(–). Translation time is 15 min. Error bars, S.D. (n = 3).