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d-amino acid incorporation into various peptide sequences promoted by EF-P. (A) mRNA sequence (mR3, mR4, mR5, mR6 and mR7) and the corresponding peptide sequence (rP3, rP4, rP5, rP6 and rP7). (B) Expression levels of peptides rP3, rP4, rP5, rP6 and rP7. tRNAPro1E2 variants were used for incorporation of d-amino acids. Numbers above the bars indicate the ratio of EF-P(+) to EF-P(–). Translation time is 15 min. Error bars, S.D. (n = 3).
