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. 2017 Nov 15;45(22):12888–12903. doi: 10.1093/nar/gkx1048

Figure 2.

Figure 2.

Measurement of SNCA 3′UTR isoforms expression in HeLa and in vitro differentiated SH-SY5Y cell lines and effect of 3′UTR length on gene reporter activity. (A) Details of the five isoforms of SNCA transcript with different lengths of the 3′UTR, ranging from 290 nt (light green) to 2.5 kb (red). (B) Percentage of each of the five SNCA transcript isoforms in HeLa cells and in vitro differentiated SH-SY5Y cells measured by gene-specific 3′end RNA sequencing. (C) Dual luciferase gene reporter assay. Scheme of the three constructs carrying the sequence of SNCA 3′UTR S (575 nt), M (1074 nt) and L (2530 nt) at the 3′ of luciferase coding sequence. (D) Firefly relative to Renilla luciferase activity of the three constructs (FL-SNCA 3′UTRS, FL-SNCA 3′UTRM and FL-SNCA 3′UTRL) compared to control empty vector FL. * P-value < 0.05 (Wilcoxon's test).