Figure 7.

Dual firefly luciferase activity of SNCA 3′ UTRs upon TIAR or ELAVL1 knockdown. We measured luciferase activities of SNCA 3′UTRs with respect to control empty vector FL. (A) TIAR KD. We observed a fold decrease of 0.63, 0.68 and 0.61 for FL-SNCA 3′UTRS, FL-SNCA 3′UTRM and FL-SNCA 3′UTRL. No significant change was instead observed for the construct carrying the sequence of the firefly luciferase gene (B). ELAVL1 KD. Mild decrease of the signal is observed for FL-SNCA 3′UTRS, FL-SNCA 3′UTRM and FL-SNCA 3′UTRL with an average fold change of 0.86, 0.88 and 0.77 respectively. * P-value < 0.05 (Wilcoxon's test).