Table 1.
Primers for quantitative reverse transcription polymerase chain reaction (qRT‐PCR)
| Gene name | Forward primer (5′‐3′) | Reverse primer (5′‐3′) | Efficiency |
|---|---|---|---|
| MHC‐I | TTGTGGAGACCAGGCCTTCAG | GAGAACCAGGCCAACAATGATG | 0.99 |
| MHC‐Ia | TTGTGGAGACCAGGCCTTCAG | AATGATGCCCATGGTGAGGAA | 0.99 |
| BoLA‐NC1 | GGATCAAGAGACGCGGATACAA | CCGCAGCCGTGCATCCACT | 0.98 |
| BoLA‐NC2 | GGGTGCGCTGATCCTCACT | CCACCCACCGCGCTGTA | 0.95 |
| BoLA‐NC3 | CCAAGGAAAGTCAACAGGAATC | AATCTCTGCCGTCGTAGGC | 0.94 |
| BoLA‐NC4 | AGCGATGACAAGAGATGCCAAGAA | CCGCACCGTCATAGGCGT | 0.96 |
| NFKB1 | CTGCTCACCACCCTCCTC | GCACTTTGTTAAGAGTTAGCAAG | 0.97 |
| RELA | CCTTTCAATGGACCCACCGA | GAGAGATGGCGTAAAGGAATAG | 0.95 |
| IRF1 | GATGCCTGTCTGTTTCGGA | TGGTGAGGGGTGGGAGCAT | 0.97 |
| CREB1 | CAGACCACTGATGGACAGCAA | TGGGGAAGACGCCATAACAACT | 0.99 |
| RFX5 | TGTATCTCTACCTTCAGCTCC | GGCAGGTGTCGGTATGCT | 0.97 |
| RFXAP | CTCAGGAAACGTCAAACTGGA | CACCACTTCTGGACTTCTTAGTAA | 0.94 |
| NFYC | TCCAAGTCCAGGGGCAGC | CTGGGCTTGACCTTGTGG | 0.97 |
| CIITA | CTGTGTCACCCGTTTCAGG | GAGATTGCCAAGGTCTTCCACA | 0.98 |
| STAT1 | TCATTTGTGGTGGAAAGACAGC | GTGCCCAGAATGTTGAACTTC | 0.97 |
| DNMT1 | TGCCTCAGTGCCTCCA | GCGTGGTTCGGAGGATCT | 0.95 |
| DNMT3A | ATGACGATGGCTATCAGTCCTA | TCTTCTTTGATGGCTGCCTG | 0.95 |
| DNMT3B | AGGACTGGAGTGTGCGTC | GAATCGCAGGGTATAACTTGG | 0.96 |
| GAPDH | GAGAAGGCTGGGGCTCACTTG | GCTGACAATCTTGAGGGTGTTG | 0.99 |
BoLA, bovine leukocyte antigen; CIITA, class II MHC transactivator; MHC, major histocompatibility complex.