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. 2017 Dec 13;12(12):e0189405. doi: 10.1371/journal.pone.0189405

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© 2017 Wan et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — The pairwise variation (V_n/n+1) was analyzed between the normalization factors NF_n and NF_n+1 by geNorm program to determine the optimal number of reference genes required for effective normalization of RT-qPCR data.