Table 2. Summary of test battery collection strategy & processing/analysis methods.

Collection Strategy	Test Battery Measure	Collection Notes	Data Processing/Analysis	Interpretation Based on Increasing Workload or Fatigue
Every Interval (4 periods)	Borg’s Rating of Perceived Exertion (6–20)	Seven body parts: Lower back, hands/arms, neck, upper back, buttock, knee, ankle.	Expressed as value between 6 and 20. Submitted to non-parametric statistical tests	Increase in rating of perceived exertion (RPE) strongly coupled to fatigue [30].
	Postural Sway	Two-minute collection duration [33]. Arms at the sides, feet shoulder width apart, toes pointing forward. Participants instructed to stand as still as possible.	Root mean square displacement amplitude calculated in the anterior-posterior direction over middle 60-seconds [33].	Increase in sway (COP RMS displacement in A-P direction) associated with fatigue [34].
	Blink Frequency	Eye blink frequency (blinks/minute) during 2-minute collection, concurrent to postural sway. Participants instructed to gaze forward at a wall target.	High-pass filtered (0.1 Hz cut-off, 4th Order Butterworth), to remove amplifier DC offset. Low-pass filtered (dual-pass, 4th Order Butterworth, 10Hz cut-off), to removed EMG-based activity. Blinks determined by a threshold criterion described in [31].	Increase in eye blink rate & duration are indices of decrement in vigilance and reduced alertness [35].
	Heart Rate and Heart Rate Variability	Expressed as the number of beats per minute during 2-minute collection, concurrent to postural sway and eye blinks. Ratio of low frequency power band (0.04 to 0.15 Hz) and high frequency power band (0.15 to 0.40 Hz)–LF/HF.	Heart Rate = Frequency count. HRV = LF/HF ratio.	Decrease in HR indicative of lowered alertness. Increase in HR related to increase workload. Increase in HRV (LF/HF ratio) associated with mental tasks and fatigue [36]
	Maximum Voluntary Contraction (MVC)	Three 5-second MVC using fabricated back/lower limb force measurement system. Participants asked to sustain maximum exertion for 3-seconds with gradual increasing/decreasing ramps. Two-minute rest between contractions.	Resultant signal of the force along x, y, z axes. Resultant signal low-pass filtered (10 Hz, dual pass, 2nd Order Butterworth). MVC force determined as the peak value of the three trials.	Cited as a direct assessment of neuromuscular fatigue. Decrease in MVC force correlated with increasing neuromuscular fatigue [32]
	Choice Reaction Time (CRT)	Ten consecutive trials. Four choices based on visual stimuli at random interval time between 1 and 4 seconds [37]. Input trial timeout of 2 seconds. Accuracy based on selection of correct choice and precision of targeting inner button. Multi-Operational Apparatus for Reaction Time (Lafayette Instrument).	Reaction time and number of errors (accuracy).	Increase reaction time and number of errors with increasing mental fatigue [38].
Pre- and Post- Session	Psychomotor Vigilance Task (PVT)	Standardized 10-minute trial. Visual stimuli presented at a variable interval of 2 to 10 seconds. Participants instructed to respond to the appearance of the LED stimulus with the thumb of their dominant hand.	Measurement parameters: %Errors (# errors committed/total number of trials), mean reaction time, reciprocal reaction time (response speed: 1/(RT/1000)), mean fastest 10% reaction time, and slowest 10% of reaction time.	PVT metrics related to lapses and psychomotor speed. Increases in errors and decrease in reaction time with increasing fatigue (time on task or sleep loss) [39]. Reciprocal transform sensitive to total and partial sleep loss.
	Purdue Pegboard Task	Two, 30-second trials for dominant and non-dominant hands, with instructions to insert pins into the pegboard, as quickly and accurately as possible. One, 60-second trial requiring assembly of pegs, two washers, and collars, using both hands as quickly and accurately as possible.	Number of inserted pins or completed assemblies.	Decrease in completed assemblies or inserted pins related to decrements in hand dexterity (fine motor) skills [40].
	Semmes Weinstein Monofilament Test	Sensory measurement taken on the sole of the dominant hand and foot. Locations marked with indelible felt tip pen at the beginning of each session. Limb supported and participant blindfolded. Starting with 2.83 monofilament, filament applied to 5 locations, varying location and time of application. Three touches considered as a single test, and participants verbally indicated the sensation of a perceived touch.	Smallest perceived monofilament diameter was recorded for each hand and foot location.	Correlation between altered plantar sensitivity and balance disorders. A reliable measure of cutaneous sensation [41].