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. 2017 Dec 13;12(12):e0189071. doi: 10.1371/journal.pone.0189071

Fig 7. Spirochetes identified from in vivo culture of monkey heart tissue.

Fig 7

The DMC-cultured heart tissue was placed in BSK and back to in vitro culture for 1 week. The culture pellets were then stained with a combination of anti-OspA and anti-OspC monoclonal antibodies, followed by a red detection antibody (Panels A,B,C) or with anti-OspA followed by a green detection antibody (Panels D,E,F). Shown are differential interference contrast (Panel A) and fluorescent detection (Panels B and C) of cultures stained with monoclonal antibodies. Panels A and B: animal IN05 (untreated); Panel C: animal IH22 (treated). Panel D is a positive control B. burgdorferi culture; Panels E and F are stained specimens from heart cultures of monkeys IK14 and IL09 (both treated), respectively.