Fig 3. Deficiency of Sirt3 in platelets increased mitochondrial ROS production.

(A) Western blot analysis using washed platelets from Sirt3-/- mice or WT mice. Photograph of a representative blot of experiments is shown. (B), (C) Flow cytometric determination of mitochondrial and cytosolic ROS in platelets. Washed platelets were incubated with thrombin (100 mU/mL) or vehicle for 30 min at 37°C in the presence of (B) MitoSox or (C) dihydrorhodamine-123. MitoSox or Rhodamine positive platelets were quantified by flow cytometry for each condition. n = 6, *P<0.05 vs WT (Student’s t tests).