Fig 1. The ROS burst of C. albicans during morphogenesis.

(A) WT strain SC5314 was grown to mid log phase in YPD media at 30°C to obtain the yeast/budding form (“yeast”). Where indicated, cells were induced to form hyphae (“Hyp”) at 37°C for 1 hr with 10% FBS, IMDM or alkaline medium. Both forms of cells were subjected to ROS analysis by luminol chemiluminescence as described in Materials and Methods. (B) WT CAIF100 or the isogenic sod4Δ/Δ sod5Δ/Δ sod6Δ/Δ strain were induced to form hyphae in alkaline medium for the indicated times and subjected to ROS measurements by lucigenin chemiluminescence. Results were recorded as relative luminescence units (RLU) as described in Materials and Methods and plotted in intervals of whole minutes.