Fig 4. The ROS burst of C. albicans morphogenesis is eliminated by fre8Δ/Δ mutations.

The indicated strains were induced to form hyphae by either 10% serum (A,C) or by IMDM, alkaline or Spider medium (B) as described in Materials and Methods and ROS formation by luminol was monitored as in Fig 1. The fre8 heterozygous “fre8Δ/+” or fre8Δ/Δ complemented with a single copy of FRE8 (“fre8Δ/ΔRes”) exhibit haploinsufficiency with regard to ROS formation. All strains were in the background of SC5314.