Skip to main content
. 2017 Dec 1;13(12):e1006763. doi: 10.1371/journal.ppat.1006763

Fig 5. C. albicans ROS production as a function of FRE8 expression.

Fig 5

(A,B) The indicated strains were induced to form hyphae with 10% FBS for 1 hour and were tested for (A) FRE8 and SOD5 mRNA by qRT-PCR or (B) ROS formation by luminol chemiluminescence as in Fig 1. (A) mRNA levels were compared to that of yeast-form cells prior to serum addition. Results represent the averages of 4 samples over two independent trials, error bars equal standard error. (C-E) The indicated strains engineered to express FRE8 from the MET3 promoter were grown to mid log phase in SC medium either containing methionine (+MET) to repress FRE8, or lacking methionine (-MET) to de-repress FRE8 expression for either 1 hour (Top) or the indicated time points (Bottom). Cells were examined for (Top) ROS production by luminol chemiluminescence and (Bottom) dark field microscopy for cell morphology at 40X magnification. Images were taken of cells following the indicated times of de-repressing FRE8 expression. All strains are in the background of SC5314.